Daily maintenance and repair of pipettes

Pipette cleaning

Regularly clean the pipette, use soapy water, 60% isopropanol, 70% ethanol and other non-corrosive detergents, then rinse with distilled water, and dry naturally. Before autoclaving, make sure the pipette is suitable for high temperatures.

Precautions

When sucking liquid, it is not suitable to rebound quickly. When sucking high-viscosity liquid, the operation should be more so. Before the liquid absorption, check whether there is any foreign matter in the nozzle; the newly installed nozzle first inhales the liquid and then drains, so after 2-3 times Carry out the test operation. When the pipette temperature is very different from the calibration, the pipette needs to be calibrated separately.

1. Forward method

Inject the solution to be transferred into a clean reagent container
A. press the button to the first stop position
B. Place the pipette nozzle at a depth of 1cm on the liquid surface and slowly release the button. After the nozzle sucks in the liquid, remove the nozzle from the liquid surface and paste it on the wall of the reagent bottle to remove excess liquid.
C. Gently press the operation button to the first stop point. After about 1 second, continue to press the operation button down to the second stop point of this operation. This operation is to discharge the solution in the mouth.
D. release the button to return to the starting position

2. Backward method

Inject the solution to be transferred into a clean reagent container
A. press the button to the second stop position
B. Place the pipette nozzle at a depth of 1cm on the liquid surface and slowly release the button. After the nozzle sucks in the liquid, remove the nozzle from the liquid surface and paste it on the wall of the reagent bottle to remove excess liquid.
C. Gently press the operation button to the first stop point to release the preset liquid. Keep the operation button at the first stop position so that a small amount of liquid that does not include the pipetting amount remains in the nozzle
D. The liquid remaining in the nozzle is discarded with the nozzle or moved to the original container.