There are some tricks in the operation of pipettes. How to select the range and control the operation of pipetting is a common problem that plagues novice operators. The following are the key points for pipettes:

Install the pipette tip:

After entering the tip at the lowermost end of the pipette handle, if operating inside the tip box, shake the pipette left or right while gently pressing down or slightly rotate the pipette (only the single pipette can be rotated) ) 1-2 seconds; if using a bulk tip, gently press the tip for 1-2 seconds while gently pressing the tip in the direction of the pipette. If this operation does not achieve the desired seal, it is necessary to check the tip and pipette.

Laboratory pipette selection range:

Overall, the available range of pipettes is 10-100% of the maximum range of pipettes.

The best advice based on operational experience is that the optimal range of the pipette is 35-100% of the maximum range of the pipette.

The pipette controls the rate of pipetting:

During the pipetting process, the pipette must slowly lower the pressure of the thumb to make the liquid rise evenly and slowly in the tip.

The laboratory pipette controls the depth and angle of the aspiration:

1. The depth of the pipette tip is required to be immersed in the depth of the pipette to ensure that the pipette is required to reach the desired pipette volume; the pipette allows the outer wall of the pipette to contact the liquid as little as possible.

2. Pipette pipetting angle: The pipette must be kept in the vertical state during pipetting.

About pipette dwell time:

Pipettes for large-scale pipetting (ml class) and viscosity is significantly higher than the pipetting of water samples, after releasing the thumb during pipetting, the tip must be allowed to stay in the liquid for 3-5 seconds before it can be removed.

Laboratory pipette storage:

After the pipette has been used, the pipette must be adjusted to the maximum range and then hung up (can be hung on a dedicated stand or attached to the metal crossbar of the lab bench). Proficiency in the use of pipettes must master the above requirements.