Immersing the tip at the appropriate depth increases accuracy by 5%. If the tip is too immersed, it will cause a lot of liquid absorption. On the contrary, if the immersion is too shallow, it will take in air.

The angle of the hand-held pipette also affects accuracy. It is guaranteed that the deviation from the vertical by 20° when aspirating or draining can improve the accuracy by 2.5%.

Rinse is a quick and simple way to increase pipetting accuracy by up to 0.2%. why? Because the liquid may form a liquid film on the inner wall of the tip, the resulting error value will be less than the theoretical value. For better pipetting accuracy, the tip can be immersed 2 to 3 times with the same sample after installing a new tip, so that the film remains fairly constant, improving pipetting accuracy and repeatability. .

Guaranteed consistency of aspiration can increase accuracy by 5%. When transferring different samples, use a consistent pipetting rhythm, the speed of pressing or releasing the button is smooth and consistent, and the force to reach the first stopping point should be consistent. Rapid or unsteady pipetting can cause liquid splashing, aerosol generation, casing contamination, and sample loss.

Good dispensing skills can also improve accuracy by 1%. Better consistency is achieved when the liquid is contacted with the tip of the container during dispensing. Slide the orifice end of the tip up the container wall to remove any remaining liquid from the orifice end. The other two techniques are equally well suited for water soluble (non-stick) fluids.