If we can prepare for pre-lubrication before using the pipette, we can avoid the above problems to the utmost! So what is pre-lubrication? Pre-lubrication means that the pipette volume should be adjusted to the maximum capacity (ie, the maximum nominal capacity) before starting to use the pipette, then press the pipette button to the lowest position and return it to the lowest position. In the initial position, repeat this process 3-6 times before starting to install the nozzle for pipetting!

why? Inside the pipette, the sealing and lubrication between the piston and the piston sleeve is achieved by special mineral grease, so if the pipette stays unused for a long time, such as staying for 2-3 days or longer When the time is not used, the silicone grease between the piston and the piston sleeve inside the pipette will have different degrees of bonding. This is like the piston and the piston sleeve. Some contact surfaces are completely covered with silicone grease and form a good layer. The film can be used for lubrication and sealing, while some contact surfaces are not, which leads to poor performance or poor sealing during operation, which affects the pipetting result!

In fact, many new pipettes have similar problems, because many new pipettes may have been stored at the manufacturer or supplier for a long time, so for those engaged in experimental research, Pre-lubrication 3-6 times before use is especially important!

In fact, relevant international technical standards (such as ISO 8655) also mention and require similar pre-lubrication preparations for pipettes before starting the operation. The above staff who wish to engage in or use the pipette can pay attention to it and apply it in daily work to form a good habit!